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KMID : 0381120160380080767
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Genes and Genomics
2016 Volume.38 No. 8 p.767 ~ p.777
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Genetic and antigenic characterization of fragments of the pheasant immunoglobulin Y heavy chain constant region
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Zhai Zhenzhen
Ma Xingliang
Yang Lingling
Ti Jinfeng
Song Daozhen
Fu Shijun
Li Dapeng
Chang Weishan
Zhai Jing
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Abstract
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Few studies have attempted to characterize the pheasant (Phasianus colchicus) immunoglobulin Y (IgY) heavy chain constant region. In the present study, fragments of the pheasant IgY heavy chain constant region were cloned, analyzed, and expressed. The cross-reactivity of IgY or immunoglobulin G (IgG)s with antigens from other vertebrate species was determined using dot-enzyme-linked immunosorbent assay and western blot analysis. Five peptides of the pheasant IgY heavy chain constant region were synthesized to determine its immunoregulatory activity in vitro. The IgY heavy chain constant region from pheasant showed the highest homology with that from chicken (71.2 %) and duck (49.1 %). Phylogenetic analysis for IgY showed that pheasant was closely related to chicken and duck than to any other analyzed vertebrate species. The rabbit anti-chicken IgG showed immunologic cross-reactivity with recombinant proteins of the pheasant IgY heavy chain constant region. Four peptides were able to induce significant up-regulation of interleukin (IL)-1β, IL-4, and interferon-γ in chicken peripheral blood lymphocytes, suggesting a new role of avian IgY in immune regulation.
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KEYWORD
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Constant region, CH-peptides, Immunoglobulin Y, Pheasant, Cross-reactivity
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